Automated, aseptic sampling with small-volume capacity from microbioreactors for cell therapy process analysis.

ABSTRACT: Current workflows in autologous cell therapy manufacturing are reliant on manual processes that are difficult to scale out to meet patient demands. High throughput bioreactor systems that enable multiple cultures to occur in parallel can address this need, but require good bioprocess monitoring workflows to produce good quality cell therapy products. Commercial sampling systems have thus been developed for better feedback control and monitoring capabilities. However, they are targeted towards large scale processes and often bioreactor specific, making them less robust for integration across different bioreactor scales and types, such as perfusion-capable microbioreactors which allows for greater process intensification. Here, an automated cell culture sampling system (Auto-CeSS) was develo

SUBMITTER: Chan ZX 

PROVIDER: S-EPMC12351187 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature