Diagnosis On-Demand: Field Evaluation of Microfluidic Paper Device for the Detection of Asymptomatic Malaria.

ABSTRACT: We report a proof-of-concept surveillance strategy that combines simplicity with modest cost to achieve high analytical performance for the detection of asymptomatic malaria infection. We developed a microfluidic paper-based analytical device (μPAD) that automates immunoassay using 30 μL of whole blood. A novel dendrimer-based signal transduction method was introduced to amplify the mass spectrometry (MS) signal. Our ability to detect an immunoassay signal with MS was achieved using cleavable ionic probes that were attached to monoclonal antibodies via dendrimer bioconjugation. The limit of detection of this MS-based immunoassay was determined to be 4.5 pM in serum for Plasmodium falciparum histidine-rich protein 2. The high stability of the ionic probes allowed storage of the paper

SUBMITTER: Seth A 

PROVIDER: S-EPMC12351687 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature