A High Efficiency Method for the Extraction and Quantitative Analysis of 45 PFAS in Whole Fish.

ABSTRACT: This study describes and validates a new method for extracting perfluoroalkyl and polyfluoroalkyl substances (PFAS) from whole-body fish tissue, demonstrates that freeze-dry preservation of tissue conserves bioaccumulative PFAS, and details a method demonstration on Lake Michigan fish. While fish filets are more commonly analyzed for their significance to human health, whole fish are useful to determine ecological impacts, but published methods such as EPA 1633 do not produce reliable results for this more challenging matrix. Here we show that lipid removal technology produces clean extracts without the need for solid-phase extraction or evaporative concentration, which often lead to loss of some PFAS. This method achieves an accuracy of 96 ± 9% for the detection of 45 PFAS while also offe

SUBMITTER: Balgooyen S 

PROVIDER: S-EPMC12351992 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature