Template-directed ligation of recognition-encoded melamine oligomers.

ABSTRACT: The templated ligation of DNA oligomers allows quantitative coupling reactions at very low concentrations and with very high selectivity. Recognition-encoded melamine oligomers (REMO) also form sequence-selective duplexes, and it should therefore be possible to ligate them in the same way. REMO duplex formation is based on H-bonding interactions between phosphine oxide (A) and 4-nitrophenol (D) recognition units, so two DDD oligomers could be ligated using an AAAOAAA template (where O represents a blank recognition unit). Two different types of chemistry were investigated: S_NAr coupling of a piperazine with a dichlorotriazine, and CuAAC coupling of an azide and an alkyne. Quantitative templated ligation was observed in the presence of competing reagents, whereas statistical mixt

SUBMITTER: Beale LA 

PROVIDER: S-EPMC12352616 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature