Concordance of molecular microbiology and conventional culture techniques for infected diabetic foot ulcer management.

ABSTRACT:

Aim

The management of infected diabetic foot ulcers (DFUs) requires balancing the need for timely interventions against the desire for targeted antibiotic therapy, which relies on laboratory results. This study aimed to evaluate concordance between molecular and conventional culture and sensitivity (C&S) methods in identifying bacteria from infected DFUs.

Methods

This study was conducted alongside CODIFI2, a Phase III randomised controlled trial comparing tissue sampling with wound swabbing. It assessed C&S and metagenomic 16S rRNA gene sequencing (M16S) in DFUs with suspected mild or moderate infections using both tissue and swab samples.

Results

In 145 participants, C&S identified 248 microorganisms across 25 genera including eight anaerobic genera. M16S identified

SUBMITTER: Oates A 

PROVIDER: S-EPMC12352717 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature