Development of an affordable multiplex quantitative RT-PCR assay for early detection and surveillance of Dengue, Chikungunya, and co-infections from clinical samples in resource-limited settings.

ABSTRACT:

Background

Dengue and Chikungunya are Aedes-borne diseases that are predominantly prevalent in tropical and subtropical regions, affecting public health globally. Dengue is caused by multiple antigenically different Dengue virus (DENV) serotypes (DENV-1 to DENV 4) in the Flaviviridae family and Chikungunya (CHIKV) in the Togaviridae family. The overlapping clinical presentation of both diseases, particularly in early infection, complicates timely and differential diagnosis. In India, diagnosis primarily relies on rapid antigen-based or ELISA-based tests, which are prone to false negatives, leading to underreported disease burden. In resource-limited settings, the absence of confirmatory diagnostics often leads to reliance on clinical symptoms and epidemiological data, increasing th

SUBMITTER: Malik MR 

PROVIDER: S-EPMC12352876 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature