The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts.

ABSTRACT: A bipolar spindle composed of microtubules and many associated proteins functions to segregate chromosomes during cell division in all eukaryotes, yet both spindle size and architecture vary dramatically across different species and cell types. Targeting protein for Xklp2 (TPX2) is one candidate factor for modulating spindle microtubule organization through its roles in branching microtubule nucleation, activation of the mitotic kinase Aurora A, and association with the kinesin-5 (Eg5) motor. Here we characterize a conserved nuclear localization sequence (NLS) motif, ¹²³KKLK¹²⁶ in Xenopus laevis TPX2, which regulates astral microtubule formation and spindle pole morphology in Xenopus egg extracts. Addition of recombinant TPX2 with this sequence mutated to AALA stimula

SUBMITTER: Pena GE 

PROVIDER: S-EPMC12353509 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature