Microbiota composition-based donor selection affects FMT efficacy in a murine colitis model.

ABSTRACT:

Background

Growing evidence links gut microbial dysbiosis to inflammatory bowel disease (IBD) pathogenesis, establishing fecal microbiota transplantation (FMT) as a microbiota-targeted therapy; however, variable outcomes in randomized trials highlight the need to identify compositional features of donor microbiota associated with FMT efficacy.

Objective

This study aimed to investigate how the composition of the donor gut microbiota influences the therapeutic efficacy of FMT in IBD.

Method

Fecal DNA from 39 IBD patients and 42 healthy donors was analyzed via 16S rRNA sequencing. Donor-enriched genera (identified through differential analysis and median abundance thresholds) guided FMT selection. Dextran sulfate sodium (DSS)-induced colitis mice received donor microbiot

SUBMITTER: Dai Z 

PROVIDER: S-EPMC12353733 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature