Structured detection for simultaneous super-resolution and optical sectioning in laser scanning microscopy.

ABSTRACT: Fast detector arrays enable an effective implementation of image scanning microscopy, which overcomes the trade-off between spatial resolution and signal-to-noise ratio of confocal microscopy. However, current image scanning microscopy approaches do not provide optical sectioning and fail with thick samples unless the detector size is limited, thereby introducing a new trade-off between optical sectioning and signal-to-noise ratio. Here we propose a method that overcomes such a limitation. From single-plane acquisition, we reconstruct an image with digital and optical super-resolution, high signal-to-noise ratio and enhanced optical sectioning. On the basis of the observation that imaging with a detector array inherently embeds axial information, we designed a straightforward reconstructio

SUBMITTER: Zunino A 

PROVIDER: S-EPMC12353846 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature