Direct visualization of chimeric antigen receptors on primary human T cells using <i>d</i>STORM super-resolution microscopy.

ABSTRACT: Chimeric antigen receptor (CAR) T cells are a transformative treatment for hematological malignancies, and concerted efforts in the field are aiming to translate this success to solid tumors and autoimmune diseases. There is a desire in the field to accurately assess CAR organization and spatiotemporal expression to elucidate mechanistic details of CAR-T cell mediated anti-tumor activity and enable evaluation of the potency and safety of CAR-T cell products. We applied an IgG4-targeted F(ab)₂ to achieve direct CAR labeling for super-resolution microscopy by direct stochastic optical reconstruction microscopy (dSTORM). This enabled us to determine CAR surface expression on human primary T cells with single-molecule resolution independent of CAR specificity. We combi

SUBMITTER: Gehrke L 

PROVIDER: S-EPMC12354347 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature