CRISPR/Cas9-mediated simultaneous targeting of GmP34 and its homologs produces T-DNA-free soybean mutants with reduced allergenic potential.

ABSTRACT: Soybean (Glycine max L.) P34 (GmP34) is a prominent allergenic seed protein belonging to the papain-like cysteine protease family. To mitigate its allergenic potential, we implemented a CRISPR/Cas9-based genome editing strategy targeting GmP34 along with its two highly similar homologs, GmP34h1 and GmP34h2, in the soybean cultivar Williams 82. Phylogenetic analysis and domain characterization identified GmP34h1 and GmP34h2 as the closest homologs to GmP34, with conserved allergenic peptide motifs. Gene expression profiling revealed similar expression patterns of all three genes during seed maturation, indicating potential functional redundancy. Two multiplex CRISPR/Cas9 constructs were designed to simultaneously target GmP34/GmP34h1 and GmP34

SUBMITTER: Baek D 

PROVIDER: S-EPMC12354366 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature