In vitro effect of fluoride-free mouthwashes on Streptococcus mutans biofilm.

ABSTRACT:

Objective

To evaluate the efficacy of commercially available, fluoride-free mouthwashes sold in Indianapolis, IN, on Streptococcus mutans biofilm.

Materials and methods

Eighty-one different mouthwashes were purchased. A 16-h culture of S. mutans UA159 was treated with the mouthwashes in three dilutions (1:3, 1:6, and 1:12), prepared in Tryptic Soy broth supplemented with 1% sucrose. The minimum inhibitory concentrations (MIC), planktonic, and biofilm growth were evaluated using a spectrophotometer. In addition, the growth for minimum bactericidal concentration (MBC) was evaluated using five μL of the dilution and incubated on blood agar. For the analysis of the results, the mouthwashes were separated into six groups according to their active ingredients (cetylpyridinium chlo

SUBMITTER: Valdivia-Tapia AC 

PROVIDER: S-EPMC12354505 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature