Characterization and Phylogenetic Analysis of the Chloroplast Genome of <i>Carissa spinarum</i> L. (Rauvolfioideae, Apocynaceae).

ABSTRACT: Carissa spinarum L. (Rauvolfioideae, Apocynaceae), a thorny shrub indigenous to arid regions of South Asia (including Pakistan), is traditionally used to treat fever, diabetes, and inflammation. This study presents the first de novo assembly of the complete chloroplast (cp) genome of C. spinarum. The genome comprises 154,654 base pairs (bp) and displays the typical quadripartite structure, consisting of a large single-copy (LSC) region (84,929 bp), a small single-copy (SSC) region (18,123 bp), and a pair of inverted repeats (IRa and IRb; 25,801 bp each). Annotation identified 113 unique genes, including 79 protein-coding genes (CDSs), 30 transfer RNAs (tRNAs), and four ribosomal RNAs (rRNAs), with 16 genes duplicated in the IRs (five CDSs, four rRNAs, and seven tRNAs). Relati

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12354977 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature