Molecular cytogenetic study on the scleractinian coral <i>Micromussaamakusensis</i> (Veron, 1990) (Hexacorallia, Anthozoa, Cnidaria): isolation of five fluorescence <i>in situ</i> hybridization markers.

ABSTRACT: Scleractinian (stony) corals are foundational to reef ecosystems, yet their taxonomy remains unresolved due to morphological plasticity and limited cytogenetic data. This study presents the first molecular cytogenetic characterization of the scleractinian coral Micromussaamakusensis (Veron, 1990), employing fluorescence in situ hybridization (FISH) to isolate and map five DNA markers. Using the conventional Giemsa staining technique, M.amakusensis was found to have a diploid karyotype of 2n = 28, with a prominent homogeneously staining region (HSR) on the long arm of chromosome 12. Subsequently, five FISH markers designated as MA-H3 for histone H3, MA-5S for 5S rRNA, MA-18/28S for 18S-28S rRNA, MA-13C for centromeric region, and MA-TEL for telomeric regio

SUBMITTER: Baldove AB 

PROVIDER: S-EPMC12355185 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature