The Use of dSTORM-Based Single Exosome Analysis To Study Tetraspanin Abundance in Extracellular Vesicles.

ABSTRACT: Serum-derived exosomes are membrane-enclosed nanovesicles secreted by cells, typically between 30 and 120 nm in diameter. Exosomes can be identified by the presence of tetraspanin protein markers including CD9, CD81, and CD63 among others. The relative amounts of these exosomal markers and their location in the exosomes are also related to their source of origin. The ability to investigate these different markers and their locations in individual and multiple exosomes was obtained using an optical imaging technique known as dSTORM (direct stochastic optical reconstruction microscopy) which can overcome the diffraction limit for detection of these nanovesicles. The use of the dSTORM imaging method has allowed us to evaluate the relative abundance of tetraspanin markers CD9, CD81, and CD63 i

SUBMITTER: Abhange K 

PROVIDER: S-EPMC12355246 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature