Advanced Extracellular Vesicle Isolation: A Hybrid Electrokinetic-Tangential Flow Filtration Approach for Improved Yield, Purity, and Scalability.

ABSTRACT: As extracellular vesicles (EVs) become increasingly important in diagnostics and therapeutics, achieving both improved purity and yield during isolation remains a critical challenge. Conventional techniques often suffer from the coisolation of nonvesicular particles and soluble proteins, limiting their clinical and research utility. In response, we introduce ExoTFF, a hybrid isolation technology that sequentially integrates electrokinetic filtration (ExoFilter) with size-exclusion tangential flow filtration (TFF) to deliver unprecedented performance gains through an iterative, synergistic mechanism. In the ExoTFF system, the sample is repeatedly circulated through an electrokinetic mesh filter and TFF until the liquid is removed. This recirculating flow gradually eliminates contaminants, w

SUBMITTER: Kim Y 

PROVIDER: S-EPMC12355454 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature