Chemical Fingerprint Imaging <i>In Planta</i> with Broadband Coherent Anti-Stokes Raman Scattering Microscopy.

ABSTRACT: Plants are inherently complex systems dynamically interacting at different size scale levels. Spontaneous Raman microscopy links the molecular with the cellular structural level; however, as Raman scattering is a low-probability phenomenon, pixel dwell times for biological applications are not compatible with high-resolution imaging. Due to absorption and autofluorescence interferences, Raman methods are often restricted to pigment-poor regions in plant samples. Here, we apply broadband coherent anti-Stokes Raman scattering (BCARS) microscopy─a nonlinear optical counterpart of spontaneous Raman microscopy─for the first time on plant samples. We show that it generates Raman-like vibrational signals but with much faster acquisition times (10 ms/spectrum), facilitating large-area imaging in h

SUBMITTER: Ebersbach P 

PROVIDER: S-EPMC12355479 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature