A novel recognition of the major allergen, Hum j 1, from <i>Humulus japonicus</i> pollen.

ABSTRACT:

Background

Humulus japonicus (HJ) is widely spread in East Asia. HJ pollen is one of the common allergens in the north of China in autumn. However, some controversy exists regarding the major allergen Hum j 1 from HJ pollen, which hamper its application in the diagnosis and treatment of HJ pollen allergy. Further studies are urgently needed to improve this aspect.

Methods

During our work in isolating potential allergens from HJ pollen, we found a protein of around 10 kDa with obvious immunoglobulin E (IgE) binding activity than other fractions. This protein was then further identified by mass spectrometry, and its gene sequence was matched from our in-house built transcripts of HJ pollen based on the identified peptides. The gene was further confirmed by polymerase ch

SUBMITTER: Li Q 

PROVIDER: S-EPMC12355532 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature