Peroxiredoxin Ⅲ mitigates mitochondrial H₂O₂-mediated damage and supports quality control in cardiomyocytes under hypoxia-reoxygenation stress.

ABSTRACT: Peroxiredoxin Ⅲ (PrxⅢ) is a mitochondria-localized peroxidase that plays a key role in detoxifying hydrogen peroxide (H₂O₂) and preserving organelle homeostasis. While its antioxidant function is well established under physiological conditions, the role of PrxⅢ in the context of cardiac hypoxia/reoxygenation (H/R) injury remains incompletely understood. In this study, we investigated the protective function of PrxⅢ in cardiomyocytes exposed to H/R stress, a widely used in vitro model to mimic ischemia/reperfusion injury. Using H9c2 cells and primary neonatal rat cardiomyocytes, we found that PrxⅢ knockdown significantly increased mitochondrial H₂O₂ accumulation, leading to excessive mitochondrial fragmentation, impaired mitophagy, and reduced cel

SUBMITTER: Park JW 

PROVIDER: S-EPMC12355573 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature