Proteomic identification of PA2146 as a biofilm marker of <i>Pseudomonas aeruginosa</i> on endoscope channel material.

ABSTRACT:

Study background and aims

Pseudomonas aeruginosa can persistently contaminate endoscopes by forming biofilms within internal channels, complicating both detection and eradication. Current microbiological surveillance methods have limited efficacy and may yield false-negative results. This study aimed to identify proteomic markers of P. aeruginosa biofilms on endoscope channel material.

Methods

Three genetically unrelated P. aeruginosa isolates from contaminated duodenoscopes and two reference strains (ATCC 27853 and PAO1) were used. Biofilms were grown on disinfected endoscope biopsy channel rings and incubated for 24, 48, and 72 h. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was employed to analyze tempora

SUBMITTER: van der Ploeg K 

PROVIDER: S-EPMC12355587 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature