Preparation of Agar Bead Embedded Mycobacterium abscessus to Inoculate Immunocompetent Mice Intratracheally.

ABSTRACT: Robust mouse models of chronic infection with Mycobacterium abscessus, an environmental pathogen that preferably infects the lungs, are badly needed. Year-long multidrug therapies deliver unacceptably poor cure rates in patients, around 50%, hence the need for predictive preclinical tools to develop better antibiotics. However, immunocompetent mice are generally resistant to pulmonary infection by M. abscessus. Numerous attempts to establish a sustained infection with M. abscessus in immunocompetent mice have been reported in the literature. Among these, methods relying on agar bead-embedded bacteria inoculated into C57BL/6 mice via the intratracheal route have proven most promising. The major limitation of this approach is the technical challenge associated with the preparation of agar be

SUBMITTER: Proietto J 

PROVIDER: S-EPMC12355601 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature