A multiplex targeted NGS panel for identifying pathogens in canine neurological and reproductive diseases.

ABSTRACT:

Introduction

Multiple pathogens can infect the canine reproductive and central nervous systems, including organisms that are zoonotic, such as Brucella canis, pathogenic Leptospira spp., Anaplasma phagocytophilum, Histoplasma capsulatum, and Blastomyces dermatitidis. In this study, we developed a targeted next-generation sequencing (tNGS) panel to identify common infectious agents related to neurologic and reproductive disease in canines while incorporating less common zoonotic agents into a single test.

Methods

Primer pools were developed to detect 34 pathogens and used in two multiplex PCR assays, which were combined prior to library preparation and sequencing using Ion Torrent technology. A feasibility study was performed with known posi

SUBMITTER: Kattoor JJ 

PROVIDER: S-EPMC12355606 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature