Hyaluronic Acid-Functionalized Highly Porous Polymeric Materials for Stem Cell Culture.

ABSTRACT: We describe the synthesis of a functional macroporous polymer material and its potential use as a scaffold to support the 3D culture of hematopoietic stem and progenitor cells (HSPCs). Glycidyl methacrylate (GMA)-based emulsion-templated porous polymers (known as polyHIPEs) were prepared by photopolymerization and subsequently surface functionalized with hyaluronic acid (HA) using Huisgen azide-alkyne cycloaddition click reaction, inferring a high degree of functionalization based on the near-quantitative nature of the reactions. Quantitative azidation of GMA-based polyHIPEs is achieved by the ring opening reaction of epoxide rings with sodium azide. Reductive amination reaction is used to end-cap HA with alkyne functionality to be later clicked onto the azidified polyHIPE surfaces. The sy

SUBMITTER: Swindell JD 

PROVIDER: S-EPMC12355691 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature