Baculovirus-mediated production and purification of ferritin nanoparticles for rift valley fever vaccine development.

ABSTRACT:

Background

Rift Valley fever (RVF) is a WHO-prioritized zoonotic, vector-borne disease with no licensed prophylaxis available for humans, highlighting the need for effective vaccine strategies. Nanoparticle-based platforms for antigen presentation offer a promising approach for vaccine development.

Results

In this work, we engineered ferritin (Ft) nanoparticles to display the immunogenic Gn domain of RVF virus (GnFt) and systematically assessed the production, purification, and physico-chemical properties of the purified nanoparticles. Baculovirus-based expression systems were evaluated in insect (Sf9, High-Five™, Tnao38, and Tnms42) and mammalian cells (HEK293 and CHO), revealing Sf9 cells as the most efficient host for producing GnFt nanoparticles. In addition, affinity-ba

SUBMITTER: Rodrigues MQ 

PROVIDER: S-EPMC12355885 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature