Utilization of the CometChip assay for detecting PAH-induced DNA bulky adducts in a 3D primary human bronchial epithelial cell model.

ABSTRACT: Polycyclic aromatic hydrocarbons (PAHs), which are formed during incomplete combustion of organic materials, may cause cancer through DNA damage mediated by formation of bulky DNA adducts from PAH reactive metabolites. The airway epithelium is a primary route of exposure for inhaled PAHs, and primary human bronchial epithelial cells (HBECs) in monolayer or organotypic cultures offer a more realistic testing scenario compared to traditional cell lines. However, lack of knowledge about their capacity to mediate DNA damage through generation of reactive chemical intermediates limits their use in quantitative studies for toxicity assessment or predictive modeling compared to in vivo studies. In this study, we explored the capacity of monolayer HBECs to generate DNA damage from metabolic activa

SUBMITTER: Colvin VC 

PROVIDER: S-EPMC12356187 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature