The influence of HIV sense and antisense transcripts on stochastic HIV transcription and reactivation.

ABSTRACT: In this study, we established dozens of single provirus-infected cellular clones offering various transcriptional phenotypes of HIV. We proposed that stochastic fluctuations in HIV transcription can appear at, at least, two levels: (1) the chromosomal landscape and (2) the in situ HIV integration site. In the former case, proviruses integrating at different genomic locations demonstrated a variety of transcriptional bursting and can be classified in noise space constructed based on the parameters associated with the coefficient of variation and using a mathematical model fitting a curve of exponential decay. In the latter case, stochastic HIV transcription can be unveiled through its phenotypic bifurcation and tended to be a pure epigenetic phenomenon: the identical provirus demonstrated f

SUBMITTER: Wiecek K 

PROVIDER: S-EPMC12356406 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature