Bumps on the Road: The Way to Clean Relaxation Dispersion Magic-Angle Spinning NMR.

ABSTRACT: Microsecond-to-millisecond motions are instrumental for many biomolecular functions, including enzymatic activity and ligand binding. Bloch-McConnell Relaxation Dispersion (BMRD) Nuclear Magnetic Resonance (NMR) spectroscopy is a key technique for studying these dynamic processes. While BMRD experiments are routinely used to probe protein motions in solution, the experiment is more demanding in the solid state, where dipolar couplings complicate the spin dynamics. It is believed that high deuteration levels are required and sufficient to obtain accurate and quantitative data. Here we show that even under fast magic-angle spinning and high levels of deuteration artifactual "bumps" in ¹⁵N R_1ρ BMRD profiles are common. The origin of these artifacts is identified a

SUBMITTER: Tatman BP 

PROVIDER: S-EPMC12356580 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature