MiR-1270 suppresses glioblastoma development by transcriptional inhibition of nuclear factor IX.

ABSTRACT:

Background

Nuclear factor IX (NFIX) promotes glioblastoma (GBM) development by inducing GBM cell migration and proliferation. However, the upstream regulatory mechanisms of NFIX in GBM remain unclear.

Methods and results

To investigate the functional role of miR-1270 in GBM, a stable miR-1270-overexpressing cell model was constructed using lentiviral transduction. The functional consequences were systematically evaluated using Transwell migration, colony formation, and CCK-8 assays, as well as western blotting and quantitative real-time polymerase chain reaction. To validate the therapeutic potential in vivo, we established orthotopic xenograft models in nude mice and longitudinally monitored tumor progression through non-invasive small animal imaging, enabling comprehensive

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12356728 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature