MALDI-TOF MS technique as a new approach for simultaneous detection and differentiation of potato virus Y strains.

ABSTRACT: Due to high variability, potato virus Y (PVY) is an excellent model for developing new virus detection and strain differentiation methods. We present a pioneering assessment using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) to identify three predominant strains of PVY: PVY^O, PVY^NTN, and PVY^N-Wi. We prepared and characterized the genomic, protein, and whole-virus samples. MALDI analysis yielded distinct spectral signatures for each strain, enabling identification. The protein extracts analyzed in the LP 2-20 kDa mode showed the highest spectral richness for N-Wi and O strains, with significant statistical differentiation (p < 0.05) across specific m/z values. RT-qPCR linear detection ranged from 6000 to 0.6 pg

SUBMITTER: Dederko P 

PROVIDER: S-EPMC12356870 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature