Antitumor power of oncolytic HSV engineered with IL-12 and IL-15.

ABSTRACT: Oncolytic herpes simplex virus type 1 (HSV-1) directly targets and destroys tumor cells while selectively infecting surrounding tumor tissue and releasing progeny viruses to continue the attack. We engineered the replicative HSV-1 vector, including the oncolytic HSV-1 (oHSV) with deleted inverted repeat and α47 gene, and then the oHSV expressing interleukin-15 (IL-15) gene and the oHSV expressing IL-15 and interleukin-12 (IL-12) gene. We evaluated the titer and growth characteristics of the oHSV-1 in Vero and cancer cell lines. Furthermore, we rigorously assessed the antitumor efficacy of HSV-1 in CT26 and B16-F10 cells, along with murine tumor models. The engineered oHSV-1 demonstrated growth patterns comparable to wild-type HSV-1. The viral titers exhibited consistency within the same tu

SUBMITTER: Zhang L 

PROVIDER: S-EPMC12357093 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature