RNA-Seq dataset of wild type and PRC2 mutant mouse embryonic stem cells undergoing ground state conversion.

ABSTRACT: Mouse embryonic stem cells (mESCs) represent an exceptional model for understanding how transcriptional responses are regulated by signalling pathways during development. Treatment with a cocktail of MEK and GSK3β inhibitors ("2i") induces ground state pluripotency, characterized by increased self-renewal, reduced DNA methylation, and uniformly high expression of pluripotency markers. Polycomb Repressive Complex 2 (PRC2) is a key developmental regulator controlling stem cell self-renewal and differentiation decisions, and altered expression of PRC2 target genes is a signature of 2i-mediated ground state conversion. Here, we generated a comprehensive RNA sequencing dataset from mESCs subjected to 2i conversion time-course across five time points between 0 and 96 h. We analysed two independe

SUBMITTER: Chapa CM 

PROVIDER: S-EPMC12357242 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature