PAXX/Ku interaction is rate limiting for repair of double-strand DNA breaks requiring end processing.

ABSTRACT: In mammalian cells, DNA double strand breaks (DSBs) are primarily repaired via classical non-homologous end joining (c-NHEJ)-one of the most essential DNA repair pathways. As NHEJ does not utilize a template, this type of repair is the default mechanism for eliminating DSBs occurring in non-cycling cells. NHEJ is a crucial process in mammals, and defects of this repair pathway often result in immunological impairment owing to failure of somatic recombination in lymphocytes and improper neuronal biogenesis. The NHEJ machinery assembles in a stepwise process at DSBs and proceeds via several key repair phases including break recognition, mediated by Ku proteins (Ku70/80 heterodimer binding to DNA), DNA ends processing, and finally DNA ligation. DNA end-bound Ku recruits the large kinase prote

SUBMITTER: Gluza J 

PROVIDER: S-EPMC12357295 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature