3D printing-biomimetic local stiff niche enhances glycolysis to boost PDAC cell stem-like phenotype via N6-methyladenosine-suppressed YAP1 mRNA decay.

ABSTRACT: Cancer stem cells (CSCs), the primary source of therapy resistance in pancreatic ductal adenocarcinoma (PDAC), exist in a dynamic equilibrium through tumor microenvironment (TME)-driven plasticity. However, the stiffness heterogeneity of TME within PDAC functions on tumor cell stem-like phenotypes remains unclear. Bioinformatics, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, of CSCs identified from spatial transcriptomic and single-cell datasets of PDAC lesions exhibited activated mechanical and glycolytic pathways. Detected by the nano-indenter, PDAC tissue exhibited significant stiffness heterogeneity (200-6000 Pa). Further, biomimetic local stiffness niches were engineered using the digital light-processing (DLP) 3D-printing technology and the

SUBMITTER: Wu D 

PROVIDER: S-EPMC12357319 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature