A novel selective medium Sucrose-Bacitracin agar 10 for accurate isolation and identification of Streptococcus mutans.

ABSTRACT:

Background

To develop and evaluate Sucrose-Bacitracin agar with 10% sucrose (SB-10), a novel selective medium designed to enhance the isolation and identification of Streptococcus mutans, a key cariogenic pathogen in dental caries.

Methods

SB-10 was compared to Mitis Salivarius Bacitracin agar with 20% sucrose (MSB-20) for its ability to support S. mutans growth, promote distinctive colony morphology, and differentiate it from other oral bacteria. Sugar particle formation was assessed using wild-type and mutant strains of S. mutans lacking glucosyltransferases B and C (GtfB and GtfC). Liquid chromatography was performed to characterize the sugar particles around colonies. The specificity and clinical utility of SB-10 were further assessed by culturing clinical saliva samples

SUBMITTER: Fang Y 

PROVIDER: S-EPMC12357436 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature