Exploring cadmium-binding proteins in Japanese scallops Mizuhopecten yessoensis: isolation and characterization.

ABSTRACT: Scallops (Mizuhopecten yessoensis) accumulate abnormal levels of cadmium (Cd) in their bodies; however, the chemical form of Cd in vivo remains unclear. In this study, Cd concentrations in various scallop tissues were measured, revealing that Cd predominantly accumulated as a water-soluble component within the midgut gland. High-performance liquid chromatography combined with the post-column tetraphenylporphyrin tetrasulfonic acid (TPPS) experiment revealed that the molecular weight of the Cd-binding substances was approximately 44 kDa. Moreover, Cd-immobilized affinity chromatography and CdS precipitation identified M. yessoensis meprin A subunit alpha-like (myMEP1A) as a Cd-binding protein. The myMEP1A gene was specifically expressed in the midgut gland. TPPS measurements indicated that

SUBMITTER: Zheng Z 

PROVIDER: S-EPMC12357810 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature