Coordination of virulence factors and lifestyle transition in Pseudomonas aeruginosa through single-cell analysis.

ABSTRACT: Pseudomonas aeruginosa, a versatile Gram-negative opportunistic pathogen, relies on multiple virulence mechanisms, including a Type III Secretion System (T3SS) and several Type VI Secretion Systems (T6SS), to establish infections. The bacterial universal second messenger cyclic di-guanylate (c-di-GMP) orchestrates the lifestyle transitions of Pseudomonas aeruginosa between motile and biofilm-associated states and influences the expression of virulence traits. While it is clear that these systems are interconnected, their precise interaction on the single-cell level has remained unclear. In this study, we use single-cell analysis to dissect the role of c-di-GMP in the heterogeneity of virulence factors in P. aeruginosa populations. Our results confirm earlier findings that on the population

SUBMITTER: Chen H 

PROVIDER: S-EPMC12357884 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature