The effect of rotenone contamination on high-resolution mitochondrial respiration experiments.

ABSTRACT:

Background

High-resolution respirometry is commonly used in skeletal muscle research and exercise science to measure mitochondrial respiratory function in both permeabilized muscle fibers and isolated mitochondria. Due to the low throughput and high cost of the most used respirometer, the Oroboros 2k (O2k), multiple experiments are often conducted within the same chamber in short succession. Despite this, no methodological consideration has been given for the potential contamination of inhibitors, used to investigate the contribution of specific complexes within the electron transport chain, between experiments.

Methods

We first assessed the potential effect of inhibitor contamination on mitochondrial respiration experiments by evaluating the ability of the currently recomme

SUBMITTER: Taylor DF 

PROVIDER: S-EPMC12358111 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature