Huangkui capsule combined with finerenone attenuates diabetic nephropathy by regulating the JAK2/STAT3 signaling pathway based on network pharmacology, molecular docking, and experimental verification.

ABSTRACT:

Introduction

Diabetic nephropathy (DN) is a serious complication of diabetes with limited therapeutic options. Although Huangkui capsule (HKC) and finerenone individually show potential in DN management, their combined mechanism remains unclear. This study aimed to explore the therapeutic effects and underlying mechanisms of HKC and finerenone combination for DN.

Methods

An integrative approach combining network pharmacology, molecular docking, kinetic simulations, and experimental validation was employed in this study. Active components of HKC and finerenone, along with their potential targets, were identified through database mining. A "drug-component-target-disease" network was constructed, and interactions with the JAK2/STAT3 pathway were validated. In vivo, DN mi

SUBMITTER: Liu X 

PROVIDER: S-EPMC12358392 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature