Sub-inhibitory concentrations of oxacillin modulate biogenesis and function of extracellular vesicles secreted by oxacillin-sensitive methicillin-resistant <i>Staphylococcus aureus</i>.

ABSTRACT:

Background

Extracellular vesicles (EVs) derived from Staphylococcus aureus (S. aureus) carry multiple components, such as toxins, antigens, and resistance determinants, whose production is influenced by exposure to β-lactam antibiotics. However, systematic studies on the effects of β-lactam antibiotics on the release and functions of EVs remain limited.

Methods

Extracellular vesicles (EVs) were isolated from the OS-MRSA (OS-200) strain by ultracentrifugation, including both EVs untreated with antibiotics and those exposed to sub-inhibitory concentrations of oxacillin (OI-EVs). Proteomic analysis was performed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The functional changes of these EVs were further assessed through erythrocyte hemolysis a

SUBMITTER: Ran Z 

PROVIDER: S-EPMC12358487 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature