Spatial transcriptomics reveals Inhba/Smad2/E2f4 axis in Lrp2^high thecal cell proliferation in androgen-induced PCOS mice.

ABSTRACT:

Background

Polycystic ovary syndrome (PCOS) is a common endocrine disorder characterized by elevated androgen levels and impaired follicular development. A hallmark of PCOS is the excessive proliferation of thecal cells (TCs), which are critical for androgen production. However, the molecular mechanisms underlying this aberrant cellular expansion remain incompletely understood.

Methods

A DHEA-induced mouse model was used to recapitulate the hormonal and ovarian features of human PCOS. Spatial transcriptomics was employed to profile gene expression in ovarian tissue at cellular resolution. Differential expression analysis, pathway enrichment, and spatial co-localization were performed to identify regulatory networks. Functional assays were conducted in cultured TCs using siRN

SUBMITTER: Luo M 

PROVIDER: S-EPMC12358492 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature