Network pharmacology and metabolomics reveal mathurameha, a Thai traditional Anti-Diabetic formula, enhances glucose metabolism through PI3K-AKT/AMPK/GLUT4 pathway modulation.

ABSTRACT: Traditional herbal formulations offer promising avenues for diabetes management by targeting multiple molecular pathways. Mathurameha (MT), a polyherbal preparation, has been historically used for its antidiabetic potential. However, its molecular mechanisms remain largely unexplored. FrE exhibited potent α-glucosidase inhibition (IC₅₀ 0.3 µg/mL) and significantly enhanced glucose uptake in L6 myotubes (3.67 ± 0.23-fold) and 3T3-L1 adipocytes (IC₅₀ 6.78 µg/mL). It also stimulated insulin secretion (1.42-fold), comparable to metformin (1.46-fold), and protected INS-1 pancreatic β-cells from H₂O₂-induced apoptosis (30.65 ± 3.54%) through partial caspase-3 inhibition. LC-MS-QTOF analysis identified 73 metabolites, including ellagic acid, kushenol A, gallic acid, arctiin, neoandrographolide, a

SUBMITTER: Banerjee S 

PROVIDER: S-EPMC12358555 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature