Split-YFP-coupled interaction-dependent TurboID identifies new functions of basal cell polarity in <i>Arabidopsis</i>.

ABSTRACT: The formation of a body axis is one of the fundamental steps in developmental patterning in multicellular organisms. Ectopic expression of the stomatal protein BASL (BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE) reveals a proximal-distal cell polarity field in the leaf and an apical-basal field in the hypocotyl and root of Arabidopsis. This provides a framework for uncovering molecular components of body-axis cell polarity in higher plants. In this study, we developed a proximity labeling-based complementation system, termed tSYID (tandem fusion of Split-YFP and TurboID), to enable simultaneous visualization and identification of the tissue-wide cell polarity module marked by interacting BASL and BREVIS RADIX-LIKE 2 (BRXL2) proteins in Arabidopsis

SUBMITTER: Huang A 

PROVIDER: S-EPMC12358837 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature