Epitope-Specific Antibody Immunodominance Driving Antibody and Influenza Viral Evolution During 2010- 2024.

ABSTRACT:

Background

Understanding hemagglutination inhibition antibody immunodominance (HAI-Ab-ID) is key to forecasting influenza virus antigenic drift and improving vaccine strain selection. We explored epitope-specific HAI-Ab-IDs in adults immunized with A/California/07/2009-like (CA/09) vaccine and A(H1N1)pdm09 viral evolutions.

Methods

Sera from adults (N = 300; birth year, 1961-1998) collected from 2010 to 2016 were analyzed in HAI assays. To determine epitope-specific HAI-Ab-IDs, 4 reverse genetics (RG) viruses were generated: RG-wt possessing CA/09 wild type hemagglutinin and 3 RG mutants containing K163Q, K130 deletion, or D127N/N129T mutations. To analyze cross-reactive or strain-specific HAI-Ab-IDs, 10 historical 1977-2007 A(H1N1) viruses were used. Antibody adsorption ass

SUBMITTER: Lu X 

PROVIDER: S-EPMC12359040 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature