Internal ribosome entry sites enhance translation in trans in antisense non-coding SINEUP and circular RNAs.

ABSTRACT: Sequences in the 5'-untranslated regions of cellular and viral mRNAs can function as internal ribosome entry sites (IRESs), driving cis-acting translation of the downstream protein-coding open reading frame. Here we demonstrate that RNA sequences with either newly identified or well-characterized IRES activity can also induce trans-acting translation of an independent mRNA species through an antisense sequence. SINEUPs are antisense long non-coding RNAs that enhance the translation of overlapping sense mRNAs in trans by employing two critical domains: the invSINEB2 sequence, which up-regulates translation (effector domain), and an antisense region providing target specificity (binding domain). First, we show that the invSINEB2 from the natural SINEUP AS Uchl1 RNA acts as an IRES when funct

SUBMITTER: D'Agostino S 

PROVIDER: S-EPMC12359042 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature