Tumour extracellular vesicle surface Protein-mRNA integration assay for early detection of epithelial ovarian cancer.

ABSTRACT:

Background

Early detection of epithelial ovarian cancer (EOC) is crucial for improving clinical outcomes. However, the sensitivity of primary serological marker cancer antigen 125 (CA125) is suboptimal for detecting early-stage EOC. Tumour-derived extracellular vesicles (EVs) are promising biomarkers for early cancer detection.

Methods

We developed an EOC EV Surface Protein-mRNA Integration (SPRI) Assay for early detection of EOC. This assay quantifies reference mRNAs within subpopulations of EOC EVs enriched by EV Click Beads targeting three EOC EV surface protein markers. Three EOC EV surface protein markers (i.e., FRα, MSLN, and TROP2) were selected through a bioinformatic framework using multi-omics data and underwent rigorous validation using EOC cell lines and EOC tiss

SUBMITTER: Yen YT 

PROVIDER: S-EPMC12359246 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature