CHH demethylation in the ZmGST2 promoter enhances maize drought tolerance by regulating ROS scavenging and root growth.

ABSTRACT: As an important food crop worldwide, maize is frequently impacted by drought stress. It is of great significance to study its drought resistance genes and mechanisms, in which DNA methylation modification in the promoter region plays a key role in regulating gene expression and stress resistance. In this study, using PEG6000 and 5-azadC to treat different drought-tolerant maize inbred lines (drought-tolerant R99 and drought-sensitive Mo17), combined with transcriptomic analysis, identified the gene ZmGST2 as being related to drought resistance. Subsequently, the methylation changes in its promoter region were further analyzed. The results indicated that the expression level of ZmGST2 was closely related to the methylation status of its promoter region under drought stress. Specifically, in

SUBMITTER: Yan X 

PROVIDER: S-EPMC12359901 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature