FACS-Sortable Triple Emulsion Picoreactors for Screening Reactions in Biphasic Environments.

ABSTRACT: Biphasic environments can enable successful chemical reactions where any single solvent results in poor substrate solubility or poor catalyst reactivity. For screening biphasic reactions at high throughput, a platform based on microfluidic double emulsions can use widely available FACS (Fluorescence Activated Cell Sorting) machines to screen millions of picoliter reactors in a few hours. However, encapsulating biphasic reactions within double emulsions to form FACS-sortable droplet picoreactors requires optimized solvent phases and surfactants to produce triple emulsion droplets that are stable over multi-hour assays and compatible with desired reaction conditions. This work demonstrates such FACS-sortable triple emulsion picoreactors with a fluorocarbon shell and biphasic octanol-in-water

SUBMITTER: Thompson S 

PROVIDER: S-EPMC12360416 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature