A pair of congenic mice for imaging of transplants by positron emission tomography using anti-transferrin receptor nanobodies.

ABSTRACT: Two anti-transferrin receptor (TfR) nanobodies, V_HH123 specific for mouse TfR and V_HH188 specific for human TfR, were used to track transplants non-invasively by PET/CT in mouse models, without the need for genetic modification of the transferred cells. We provide a comparison of the specificity and kinetics of the PET signals acquired when using nanobodies radiolabeled with ⁸⁹Zr, ⁶⁴Cu, and ¹⁸F, and find that the chelation of the ⁸⁹Zr and ⁶⁴Cu radioisotopes to anti-TfR nanobodies results in radioisotope release upon endocytosis of the radiolabeled nanobodies. We used a knock-in mouse that expresses a TfR with a human ectodomain (Tfrc^hu/hu) as a source of bone marrow for transplants into C57BL/6 r

SUBMITTER: Balligand T 

PROVIDER: S-EPMC12360783 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature