Discordant effects of maternal age on the human MII oocyte transcriptome.

ABSTRACT: While advanced maternal age is associated with significant changes in oocyte gene expression, these are not global changes but limited to a fraction of the transcriptome. However, there is little consensus on the specific genes affected, and on the transcriptomic signatures of age-related declines in oocyte quality. To characterize the effects of age on the human MII oocyte transcriptome, here we take a two-part approach. We first generated single-oocyte Smart-seq2 datasets from 10 younger (21-29 years) and 10 older (37-43 years) donors, identifying genes differentially expressed between the two groups, then cross-referenced our results with those of 12 studies (9 human, 3 mouse) performing equivalent analyses using a variety of single-cell transcriptomic or microarray platforms. Technical

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12360849 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature