Hidden Markov Model-Based Prokaryotic Genome Space Mining Reveals the Widespread Pervasiveness of Complex I and Its Potential Evolutionary Scheme.

ABSTRACT: Most cellular reactions are interdependent; however, a subset of reactions often associate more closely to form a defined reaction pathway. An extreme arrangement of interdependent reactions occurs when the cognate proteins physically associate to constitute a complex. Respiratory Complex I (C-I) is one of the largest membrane-resident protein assemblies. Besides being a hallmark of bioenergetics, this enzyme complex is critical for maintaining redox homeostasis and facilitating transport. However, its evolutionary origins are unclear due to challenges in identifying close homologs and subunit ancestry. Using custom hidden Markov model (HMM) profiles, we analyzed the prokaryotic genome space to trace the distribution of 14 core C-I or NADH-quinone oxidoreductase (Nuo) subunits. Our finding

SUBMITTER: Shirsath A 

PROVIDER: S-EPMC12361115 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature